superscript ii rnase h 2 reverse transcriptase (Thermo Fisher)
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superscript ii rnase h 2 reverse transcriptase
Superscript Ii Rnase H 2 Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+ii+rnase+h+2+reverse+transcriptase/Ribonuclease+A/pmc12664216-50-17-24
Average 99 stars, based on 1 article reviews
Superscript Ii Rnase H 2 Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+ii+rnase+h+2+reverse+transcriptase/Ribonuclease+A/pmc12664216-50-17-24
Average 99 stars, based on 1 article reviews
superscript ii rnase h 2 reverse transcriptase - by Bioz Stars,
2026-10
99/100 stars
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Synthesized:Article Title: Lung disease associated with the IVS8 5T allele of the CFTR gene. Article Snippet: The cells were immediately washed in F12 medium, and lysed in 1 ml of TRIzol reagent for RNA extraction (GIBCO BRL, Bethesda, MD). cDNA synthesis . .. The first strand of cDNA was synthesized by using oligo(dT) 12–18 and Article Title: Integrative Signaling by Minimal Erythropoietin Receptor Forms and c-Kit Article Snippet: Northern Blotting, RNase Protection, RT-PCR, and cDNA Array Assays—RNA was isolated using TRIzol reagent (Life Technologies, Inc.) and Northern blotting was performed as described (26) using the following 32P-labeled murine cDNA probes: cis (1-kb EcoRI to NotI fragment of pCRV) (5), c-Myc (1.5-kb XhoI fragment of pSVLc-Myc) (29), bcl-xL (0.8-kb EcoRI fragment of pBlueScriptSK(1)bcl-xL) (24), Epo receptor (1.5-kb XhoI fragment of pXMwtER) (30), pim-1 (1.3-kb EcoRI to XbaI fragment of pCMP2D3) (23), SOCS-3 (0.5-kb SmaI to XbaI fragment of pEF-SOCS-3) (22), and GAPDH (0.8-kb KpnI to XhoI fragment of pSP-GAPDH). .. RNase protection assays were performed using a RiboQuant multi-probe system according to the manufacturer (PharMingen, San Diego, CA). cDNAs for PCR analysis were synthesized using an oligo(dT) primer (Life Technologies, Inc.) and Article Title: Characterization of a Sabin-like type 2/3 recombinant poliovirus with a triple nucleotide deletion in the VP1 region isolated from sewage Article Snippet: Viral RNA was extracted from poliovirus isolates using the QIAamp Mini Viral RNA Extraction Kit (Qiagen) and subsequently used for RT-PCR amplification, following standard protocols [ ]. .. Single-stranded cDNA was synthesized from 5 μL of purified viral RNA using 1 μL (200 U) of Article Title: Hepatitis C virus core protein genotype 3a increases SOCS-7 expression through PPAR-{gamma} in Huh-7 cells. Article Snippet: .. Total RNA was extracted from Huh-7 cells transiently expressing HCV core protein genotype 3a or transfected with empty vector (pIRES2-EGFP) by using an RNeasy Mini kit (Qiagen) and subsequently digested by DNase I. cDNA was synthesized from 100 ng total RNA with Reverse Transcription:Article Title: Lung disease associated with the IVS8 5T allele of the CFTR gene. Article Snippet: The cells were immediately washed in F12 medium, and lysed in 1 ml of TRIzol reagent for RNA extraction (GIBCO BRL, Bethesda, MD). cDNA synthesis . .. The first strand of cDNA was synthesized by using oligo(dT) 12–18 and Article Title: Expression of oncofetal RNA-binding protein CRD-BP/IMP1 predicts clinical outcome in colon cancer. Article Snippet: RNA isolation and quantitative RT-PCR analysis RNA for LightCycler quantitative mRNA expression analysis was isolated using the Total RNA Isolation NucleoSpin RNA II Kit (Macherey-Nagel, Easton, PA) according to the manufacturer’s protocol. .. Total RNA samples were reverse transcribed into cDNA using approximately 1 lg of total RNA, random primers, and the Article Title: Integrative Signaling by Minimal Erythropoietin Receptor Forms and c-Kit Article Snippet: Northern Blotting, RNase Protection, RT-PCR, and cDNA Array Assays—RNA was isolated using TRIzol reagent (Life Technologies, Inc.) and Northern blotting was performed as described (26) using the following 32P-labeled murine cDNA probes: cis (1-kb EcoRI to NotI fragment of pCRV) (5), c-Myc (1.5-kb XhoI fragment of pSVLc-Myc) (29), bcl-xL (0.8-kb EcoRI fragment of pBlueScriptSK(1)bcl-xL) (24), Epo receptor (1.5-kb XhoI fragment of pXMwtER) (30), pim-1 (1.3-kb EcoRI to XbaI fragment of pCMP2D3) (23), SOCS-3 (0.5-kb SmaI to XbaI fragment of pEF-SOCS-3) (22), and GAPDH (0.8-kb KpnI to XhoI fragment of pSP-GAPDH). .. RNase protection assays were performed using a RiboQuant multi-probe system according to the manufacturer (PharMingen, San Diego, CA). cDNAs for PCR analysis were synthesized using an oligo(dT) primer (Life Technologies, Inc.) and Article Title: Characterization of a Sabin-like type 2/3 recombinant poliovirus with a triple nucleotide deletion in the VP1 region isolated from sewage Article Snippet: Viral RNA was extracted from poliovirus isolates using the QIAamp Mini Viral RNA Extraction Kit (Qiagen) and subsequently used for RT-PCR amplification, following standard protocols [ ]. .. Single-stranded cDNA was synthesized from 5 μL of purified viral RNA using 1 μL (200 U) of Article Title: Partial correction of murine hemophilia A with neo-antigenic murine factor VIII. Article Snippet: We have previously reported a factor VIII knockout (FVIII KO) mouse model for hemophilia A.. Here we demonstrate the presence of nonfunctional heavy chain factor VIII protein in the mouse, making it an excellent model for cross-reacting material (CRM)-positive hemophilia A patients, who express normal levels of a dysfunctional FVIII protein.. We attempted to correct these mice phenotypically by transduction of wild-type mouse factor VIII cDNA delivered in an E1/E3-deleted adenoviral vector by tail vein injection. Article Title: Novel concentration method for the detection of norovirus and sapovirus from water using minute particles of amorphous calcium phosphate. Article Snippet: RNAs were extracted from 140 ml from each of the FCV culture fluids or ACPdissolved solution samples obtained as described above, using a QIAamp viral RNA mini kit (Qiagen) according to the manufacturer’s instruction, except 60 ml diethyl-pyrocarbonate-treated water was used for RNA elution from the spin column. .. The reaction mixture for cDNA synthesis (30 ml) contained 15 ml extracted RNA, 0.5 mM each dNTP, 5 mM DTT, 2.5 mM random hexamers, 30 units RNasin RNase inhibitor (Promega), 300 units Article Title: Hepatitis C virus core protein genotype 3a increases SOCS-7 expression through PPAR-{gamma} in Huh-7 cells. Article Snippet: .. Total RNA was extracted from Huh-7 cells transiently expressing HCV core protein genotype 3a or transfected with empty vector (pIRES2-EGFP) by using an RNeasy Mini kit (Qiagen) and subsequently digested by DNase I. cDNA was synthesized from 100 ng total RNA with Article Title: Presenilin 1 negatively regulates beta-catenin/T cell factor/lymphoid enhancer factor-1 signaling independently of beta-amyloid precursor protein and notch processing. Article Snippet: Total RNA was extracted with Trizol (Life Technologies). .. Reverse Transcription (RT) PCR (25 ng total RNA/reaction) was performed using Polymerase Chain Reaction:Article Title: Integrative Signaling by Minimal Erythropoietin Receptor Forms and c-Kit Article Snippet: Northern Blotting, RNase Protection, RT-PCR, and cDNA Array Assays—RNA was isolated using TRIzol reagent (Life Technologies, Inc.) and Northern blotting was performed as described (26) using the following 32P-labeled murine cDNA probes: cis (1-kb EcoRI to NotI fragment of pCRV) (5), c-Myc (1.5-kb XhoI fragment of pSVLc-Myc) (29), bcl-xL (0.8-kb EcoRI fragment of pBlueScriptSK(1)bcl-xL) (24), Epo receptor (1.5-kb XhoI fragment of pXMwtER) (30), pim-1 (1.3-kb EcoRI to XbaI fragment of pCMP2D3) (23), SOCS-3 (0.5-kb SmaI to XbaI fragment of pEF-SOCS-3) (22), and GAPDH (0.8-kb KpnI to XhoI fragment of pSP-GAPDH). .. RNase protection assays were performed using a RiboQuant multi-probe system according to the manufacturer (PharMingen, San Diego, CA). cDNAs for PCR analysis were synthesized using an oligo(dT) primer (Life Technologies, Inc.) and Article Title: Presenilin 1 negatively regulates beta-catenin/T cell factor/lymphoid enhancer factor-1 signaling independently of beta-amyloid precursor protein and notch processing. Article Snippet: Total RNA was extracted with Trizol (Life Technologies). .. Reverse Transcription (RT) PCR (25 ng total RNA/reaction) was performed using Purification:Article Title: Characterization of a Sabin-like type 2/3 recombinant poliovirus with a triple nucleotide deletion in the VP1 region isolated from sewage Article Snippet: Viral RNA was extracted from poliovirus isolates using the QIAamp Mini Viral RNA Extraction Kit (Qiagen) and subsequently used for RT-PCR amplification, following standard protocols [ ]. .. Single-stranded cDNA was synthesized from 5 μL of purified viral RNA using 1 μL (200 U) of cDNA Synthesis:Article Title: Characterization of a Sabin-like type 2/3 recombinant poliovirus with a triple nucleotide deletion in the VP1 region isolated from sewage Article Snippet: Viral RNA was extracted from poliovirus isolates using the QIAamp Mini Viral RNA Extraction Kit (Qiagen) and subsequently used for RT-PCR amplification, following standard protocols [ ]. .. Single-stranded cDNA was synthesized from 5 μL of purified viral RNA using 1 μL (200 U) of Article Title: Partial correction of murine hemophilia A with neo-antigenic murine factor VIII. Article Snippet: We have previously reported a factor VIII knockout (FVIII KO) mouse model for hemophilia A.. Here we demonstrate the presence of nonfunctional heavy chain factor VIII protein in the mouse, making it an excellent model for cross-reacting material (CRM)-positive hemophilia A patients, who express normal levels of a dysfunctional FVIII protein.. We attempted to correct these mice phenotypically by transduction of wild-type mouse factor VIII cDNA delivered in an E1/E3-deleted adenoviral vector by tail vein injection. Article Title: Novel concentration method for the detection of norovirus and sapovirus from water using minute particles of amorphous calcium phosphate. Article Snippet: RNAs were extracted from 140 ml from each of the FCV culture fluids or ACPdissolved solution samples obtained as described above, using a QIAamp viral RNA mini kit (Qiagen) according to the manufacturer’s instruction, except 60 ml diethyl-pyrocarbonate-treated water was used for RNA elution from the spin column. .. The reaction mixture for cDNA synthesis (30 ml) contained 15 ml extracted RNA, 0.5 mM each dNTP, 5 mM DTT, 2.5 mM random hexamers, 30 units RNasin RNase inhibitor (Promega), 300 units Incubation:Article Title: Characterization of a Sabin-like type 2/3 recombinant poliovirus with a triple nucleotide deletion in the VP1 region isolated from sewage Article Snippet: Viral RNA was extracted from poliovirus isolates using the QIAamp Mini Viral RNA Extraction Kit (Qiagen) and subsequently used for RT-PCR amplification, following standard protocols [ ]. .. Single-stranded cDNA was synthesized from 5 μL of purified viral RNA using 1 μL (200 U) of Expressing:Article Title: Hepatitis C virus core protein genotype 3a increases SOCS-7 expression through PPAR-{gamma} in Huh-7 cells. Article Snippet: .. Total RNA was extracted from Huh-7 cells transiently expressing HCV core protein genotype 3a or transfected with empty vector (pIRES2-EGFP) by using an RNeasy Mini kit (Qiagen) and subsequently digested by DNase I. cDNA was synthesized from 100 ng total RNA with Transfection:Article Title: Hepatitis C virus core protein genotype 3a increases SOCS-7 expression through PPAR-{gamma} in Huh-7 cells. Article Snippet: .. Total RNA was extracted from Huh-7 cells transiently expressing HCV core protein genotype 3a or transfected with empty vector (pIRES2-EGFP) by using an RNeasy Mini kit (Qiagen) and subsequently digested by DNase I. cDNA was synthesized from 100 ng total RNA with Reverse Transcription Polymerase Chain Reaction:Article Title: Presenilin 1 negatively regulates beta-catenin/T cell factor/lymphoid enhancer factor-1 signaling independently of beta-amyloid precursor protein and notch processing. Article Snippet: Total RNA was extracted with Trizol (Life Technologies). .. Reverse Transcription (RT) PCR (25 ng total RNA/reaction) was performed using |